detection of metallo-beta lactamases among carbapenem-resistant pseudomonas aeruginosa

نویسندگان

ahmad farajzadeh sheikh department of microbiology, ahvaz jundishapur university of medical sciences, ahvaz, ir iran; cellular and molecular research center, ahvaz jundishapur university of medical sciences, ahvaz, ir iran

soodabeh rostami department of microbiology, ahvaz jundishapur university of medical sciences, ahvaz, ir iran; cellular and molecular research center, ahvaz jundishapur university of medical sciences, ahvaz, ir iran; department of microbiology, ahvaz jundishapur university of medical sciences, ahvaz, ir iran tel: +98-6113367543, fax: +98-6113332036

abbas jolodar department of biomolecular and biochemistry, school of veterinary medicine, shahid chamran university, ahvaz, ir iran

mohammad amin tabatabaiefar department of genetic, ahvaz jundishapur university of medical sciences, ahvaz, ir iran

چکیده

conclusions epidemiological and regional evaluation of mbl-producing p. aeruginosa through simple and inexpensive methods should be considered for effective treatment of carbapenem-resistant p. aeruginosa infections. results amongst all the p. aeruginosa isolates, 58.7% were resistant to imipenem while 31.8%, 13.5% and 74.4% were resistant to meropenem, doripenem and ertapenem, respectively. amongst all the p. aeruginosa isolates, 44.4% were multidrug resistant and 13.45% were resistant to all of the carbapenems. the cd test with doripenem disk / 750 μg ethylene diamine tetra acetic acid (edta) had the highest efficiency compared to the other phenotypic tests. blaimp and blavim genes were detected in 11.7% and 0.4% of isolates, respectively. blaspm and blandm genes were not observed. background carbapenems are important drugs used for the treatment of pseudomonas aeruginosa infections, however metallo-β-lactamases (mbl) are able to efficiently hydrolyze these classes of drugs. immediate detection of the mbl-producing p. aeruginosa is necessary in order to accurately treat infections caused by this organism. objectives to determine the prevalence of mbl producing p. aeruginosa in burn and non-burn patients by two phenotypic tests and polymerase chain reaction (pcr) and to compare phenotypic tests with pcr. materials and methods a total of 223 non-duplicate strains of p. aeruginosa were collected from three teaching hospitals of ahvaz, iran. antimicrobial susceptibility and minimum inhibitory concentrations (mics) of carbapenems (imipenem, meropenem, doripenem and ertapenem) were determined by the kirby-bauer and e-test methods. combined disk (cd) test, mbl e-test and pcr were performed for carbapenem-resistant p. aeruginosa isolates.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Detection of Metallo-Beta Lactamases Among Carbapenem-Resistant Pseudomonas aeruginosa

BACKGROUND Carbapenems are important drugs used for the treatment of Pseudomonas aeruginosa infections, however metallo-β-lactamases (MBL) are able to efficiently hydrolyze these classes of drugs. Immediate detection of the MBL-producing P. aeruginosa is necessary in order to accurately treat infections caused by this organism. OBJECTIVES To determine the prevalence of MBL producing P. aerugi...

متن کامل

Metallo‐beta‐lactamases among imipenem‐resistant Pseudomonas aeruginosa in a brazilian university hospital

INTRODUCTION Imipenem-resistant Pseudomonas aeruginosa resulting from metallo-β-lactamases has been reported to be an important cause of nosocomial infection and is a critical therapeutic problem worldwide, especially in the case of bacteremia. OBJECTIVES To determine the frequency of metallo-β-lactamases among imipenem-resistant Pseudomonas aeruginosa isolates and to compare methods of pheno...

متن کامل

IMPs, VIMs and SPMs: the diversity of metallo-beta-lactamases produced by carbapenem-resistant Pseudomonas aeruginosa in a Brazilian hospital.

Pseudomonas aeruginosa isolates (n=183), collected from bacteraemic patients hospitalised in Sao Paulo Hospital (Brazil) during 2000-2001, were screened for susceptibility to antimicrobial agents. The polymyxins were the most active compounds (100% susceptibility), followed by amikacin and cefepime (59.0%), meropenem (57.4%), and imipenem and gentamicin (55.2%). Imipenem-resistant isolates were...

متن کامل

Detection of Metallo-β-Lactamases (MBLs) Producing Pseudomonas aeruginosa Isolates in Tehran Hospitals, Iran

Introduction: The strains of Pseudomonas aeruginosa are known as an opportunistic pathogen that can cause infections in humans and animals. Metallo-β-lactamases (MBLs) are the most significant factors of resistance to carbapenem antibiotics in these bacteria. This study was designed to identify the MBLs producing P. aeruginosa isolates in three hospitals of Tehran, Iran. Methods: Tota...

متن کامل

Detection of Different Types of Metallo-β-Lactamases among Pseudomonas aeruginosa Isolates Obtained from Intensive Care Unit Patients

Introduction: Pseudomonas aeruginosa is a serious challenge for antimicrobial therapy, due to chromosomal mutations or intrinsic resistance to various antimicrobial agents, such as Metallo-β-Lactams (MBL). This study aimed to investigate the prevalence of β-lactamases encoding genes among P. aeruginosa strains isolated from intensive care unit (ICU) patients by phenotypic and multiplex PCR meth...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
jundishapur journal of microbiology

جلد ۷، شماره ۱۱، صفحات ۰-۰

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023